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Inne
Protocol of proceedings with Fusobacterium nucleatum and optimization of ABTS method for detection of reactive oxygen species.
Autorzy
Rok wydania
2020
Czasopismo
Numer woluminu
15
Strony
259-271
DOI
10.2217/fmb-2019-0010
Kolekcja
Język
Angielski
Typ publikacji
Artykuł
Aim: Characterization of the ability of Fusobacterium nucleatum DSM 15643 and DSM 20482 strains in the presence of Cu2+ and H2O2 to reactive oxygen species generation. Method: Spectrophotometric ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) method was used. Results: Determination of: MIC for Cu2+, H2O2 and ABTS; survivability of F. nucleatum under atmospheric oxygen exposure; the level and rate constants of free radicals production by the bacteria. Conclusion:F. nucleatum in the presence of Cu2+ and H2O2 is able to generate free radicals. Reactive oxygen species are produced mainly outside the bacterial cell, which suggests that outer membrane proteins may be involved in oxidative process.
Słowa kluczowe
ABTS, copper ions, fenton-like reaction, Fusobacterium nucleatum, oxidative stress
Adres publiczny
http://dx.doi.org/10.2217/fmb-2019-0010
Strona internetowa wydawcy
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